3/3/2023 0 Comments Decorar fotos pizap![]() ![]() Text styles like outline, drop shadow and text bubbles Get creative with your photo design! Enjoy these fun graphic art features: ![]() Put together motivational quotes, sayings & tips Whip up a Facebook, LinkedIn or Twitter header image in minutes Create fun and engaging social media posts All your social media design tools in one easy-to-use design app! Resize your projects to fit all popular social media & print sizes with the click of a button Print your designs in a snap with print ready layouts Choose from hundreds of fonts and text styles to customize your creation Create flyers, social media posts and so much more! With 1000’s of premade templates you can get started fast and skip the blank paper stage PROFESSIONAL GRADE GRAPHIC DESIGN MADE EASY Discover over 100 photo filters & effects Choose from over 1.8 million royalty free stock images Adding dimension to your photos is a breeze with our photo layering tool Use the paint tool & graphics to create a masterpiece Apply frames & borders to your photos for a personalized touch Adjust the colors & tones of your photos with just a few taps Smooth over imperfections with touch up & photo retouching Apply unique filters for a perfect social media photo layout Use photo crop tools to capture all you want and get rid of everything you don’t You don’t need to be a Photoshop expert to get up and running quickly with piZap Design photos & graphics for flyers, websites, presentations, or create your own emojis with ease Everything from Instagram to LinkedIn, you can design posts for all social media formats Meme Maker: Memes are fun! Why not create your very own? With piZap, you can! Social Media Photo Editor: Edit photo masterpieces for all of your favorite social channels Photo Collage Maker: Create a custom photo grid with 1000’s of unique layouts to choose from There are literally thousands of ways you can create an image with piZap’s Design Tools in a snap! Say goodbye to complicated design and photo layout software and say hello to your all-in-one picture editor that’s just as easy to use as it is fun! What will you create next?ĭESIGN ANYTHING – SOCIAL POSTS, COLLAGES, MEMES & MORE Professional-grade photo editing is at your fingertips with piZap. Best of all, most of our tools and features come at no cost to you. Whip up a viral post, stories and memes, edit and enhance photos, put together an amazing photo grid and so much more! piZap has loads of fonts, stickers, borders, filters, and stock images to choose from to help you create a design that’s uniquely your own. ![]() Create a photo layout that’s sure to turn some heads with piZap. ![]()
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3/3/2023 0 Comments Kayak perception swifty![]() ![]() Polyethylene will become more flexible when in warm conditions such as a hot, sunny day. A minimal amount of care in storage and transporting will help the kayak maintain a like-new condition for many years. Our “roto” kayaks are made of high-density polyethylene, which is virtually maintenance-free. ![]() Inflatable kayaks – which are more lightweight by design – are most commonly made of PVC.Hard-shell kayaks are usually constructed out of high density polyethylene, a type of hard, durable, and often UV-resistant plastic.For other paddlers, the Drift 9.5 weighs 40 lbs. What is the lightest Perception kayak? The lightest Perception kayak in their current lineup is the Prodigy XS, for kids and petite paddlers. With a generous capacity of 350 pounds, it features intuitive rudder control to turn on its axis and navigate tight waterways with ease. Leading the new line-up, the streamlined Mirage iTrek 9 Ultralight is Hobie’s lightest, most transportable and most maneuverable pedal kayak. Are Perception kayaks made by Wilderness Systems?Ĭonfluence Outdoor is the result of the blending of eight unique watersports brands: Wilderness Systems, Perception, Dagger, and Wave Sport kayaks, Mad River Canoe, Adventure Technology paddles, Bomber Gear and Harmony accessories. We’re a company of passionate kayakers that proudly design, mold and hand-assemble every Perception kayak in Greenville, South Carolina. However, in the recreational category of kayak, we find a 14 foot boat that weighs 45 pounds and one that weighs about 87 lbs.įor over 40 years, Perception has introduced more people to paddling than probably any other kayak maker. How much does a 14 foot kayak weigh?įor example, a 14-foot boat would be expected to weigh 68 lbs. It’s unsurpassed in terms of stability, straight line speed and tracking capabilities, with even the shorter 10.0 cruising at enough speed to cover long days on flat water. Perception Pescador – Not only is the Pescador a great fishing platform, but it’s arguably Perception’s best sit-on-top recreational kayak. You could say that, on average, kayaks weigh somewhere between 20 and 80 pounds, with some getting close to – or going beyond – the 100-pound mark. SPECS: Length: 11’5″ / 348 cm Width: 30.25″ / 77 cm Boat Weight: 52 lbs / 24 kg Max Capacity: 350 lbs / 159 kg Deck Height: 16″ / 41 cm Cockpit Length: 53″ / 135 cm Cockpit Width: 19″ / 48 cm FEATURES: Comfort Seating System (CSS) Molded-In Footwells 6″ Deck Plate w/ Catbag Tankwells w/ Bungee Molded-In Side Handles How much does the average kayak weigh? What is a Perception Swifty kayak made of? How much does a Perception kayak weigh? ![]() 3/3/2023 0 Comments Hidden brain![]() I was actually working at The Philadelphia Inquirer, at the time, my first newspaper job. ![]() I found myself a little less so, and so I gravitated to science journalism almost from the start of my career. Many journalists are highly phobic of anything to do with mathematics. It allowed me the ability to understand how scientific papers were being written. ![]() Vedantam: My background as an engineer helped me in some ways enter science journalism, Kim. How did you find your way to reporting on behavioral science and what has made you stick with it all these years? One item from your CV that I didn’t mention a moment ago is that you have an undergraduate degree in electronics engineering and a master’s degree in journalism. Mills: Let’s start with a little bit more about your story. And in the interest of full disclosure, I will tell you, APA has worked with Shankar over the years, occasionally referring him to expert psychologists as part of his reporting. He has also written plays and a collection of short stories. He is the author of two nonfiction books, Useful Delusions: The Power and Paradox of the Self-Deceiving Brain, published in 2021 and The Hidden Brain: How Our Unconscious Minds Elect Presidents, Control Markets, Wage Wars, and Save Our Lives, published in 2010. Before that he spent 10 years as a reporter and columnist at The Washington Post. He was National Public Radio’s social science correspondent from 2011 until 2020. Shankar Vedantam has been reporting on human behavior and social science research for more than 25 years. Recent episodes have covered the psychology of money, the rise of rudeness, and resilience and coping with grief, to name just a few topics. Its mission is to explore the unconscious patterns that drive human behavior. Hidden Brain is also broadcast on more than 400 public radio stations across the country. I’m talking about Shankar Vedantam, the host and executive editor of Hidden Brain, one of the most popular science podcasts in the US, which receives millions of downloads every week. Many of you probably know his work, especially if you subscribe to other science podcasts and if you listen regularly to National Public Radio. Today, I will be talking with a journalist and podcast host who has spent more than two decades bringing psychological and behavioral research to the public. ![]() This week, we’re doing something a little bit different from our usual conversation with a psychologist about his or her thought-provoking research. Kim Mills: Hello, and welcome to Speaking of Psychology, the flagship podcast of the American Psychological Association that examines the links between psychological science and everyday life. ![]() 3/2/2023 0 Comments Check errorprovider for errors![]() ![]() NET Framework 4.5.2 (Offline Installer) for Windows Vista SP2, Windows 7 SP1, Windows 8, Windows 8.1, Windows Server 2008 SP2, Windows Server 2008 R2 SP1, Windows Server 2012 and Windows Server 2012 R2 from Official Microsoft Download Centerĭownload Microsoft. Net Framework 4.5.2 installed and activated on this computer! I have also attempted to install the latest version of. I have double checked that i have the latest.I have attempted several things to get this working: Pastebin is a website where you can store text online for a set period of time. is the number one paste tool since 2002. Here is the Swarm Agent Log file after reproducing the issue! Pastebin Swarm Agent Log. Im working and rendering on the same computer. Im not trying to have Swarm Agent connect to anything else. NOTE: Reproduced using a Single computer. NOTE: This was reproduced using the default blueprint first person shooter example map Swarm Agent clears the current log, and outputs: 13:27:59: Local connection establishedġ3:27:59: Error: Provider load failure I then proceed to hit Build inside Unreal Engine 4.10. initialization successful, SwarmAgent now running initializing local performance monitoring subsystemġ3:27:42. SwarmCoordinator failed to be initializedġ3:27:41. using SwarmCoordinator on RENDER-01ġ3:27:41. initializing connection to SwarmCoordinatorġ3:27:39. recreating SwarmAgent cache staging areaġ3:27:39. using cache folder 'D:\Program Files\Epic Games\4.10\Engine\Binaries\DotNET/SwarmCache'ġ3:27:39. registering SwarmAgent network channelsġ3:27:39. registering SwarmAgent with remoting serviceġ3:27:39. Before i do so, i make sure any other instances of Swarm Agent, and Swarm Coordinator is closed, and then i manually open SwarmAgent.exe, and open the Log tab, which outputs: 13:27:39: Starting up SwarmAgent. Im gonna reproduce the issue with the default Blueprint FirstPerson Shooter Example Project, by placing a light in the Example Map, and hitting build. All Swarm Agent settings are default after a fresh reinstall. Im not trying to get Swarm Agent to connect to anything. Its a long time ago when UE4 was a paid subscription based software. I did not have this issue before, i dont know which version it was, or what happened. ![]() Im attempting to build the lighting for One computer only, which is the one im working on. ![]() I can open Swarm Agent just fine by double clicking on it. Swarm Agent is opened, as i can see the icon in the taskbar. When i attempt to build any project in Unreal Engine 4.10 the build finishes quickly with Navigation build completed, it does not start building the lighting, not even if i build lighting only. Lighting build fails, Swarm Agent outputs: Error: Provider load failure ![]() I’m really hoping someone in here can help with it! Long allbytes = new FileInfo(path).Length įileByte = br.Hi! I’ve had this issue for a long time now, but i haven’t been able to find a solution for it online, so far! string filename = (("\\") 1, - (("\\") 1)) įileStream fs = new FileStream(path, System.IO.FileMode.Open, System.IO.FileAccess.Read) below 10 lines convert image into binary data String query = "insert into arms_records (thana,licence_number,sanction_date,licencee_name,father_name,dob,religion,caste,address,village,uid,mobile,email,arms_category,type_of_arm,no_of_arms,arms_number,valid_area,renewal_date,remark,) values cmd = new OleDbCommand(query, textBoxRemark.Text) OleDbConnection con = new OleDbConnection(connectionString) String connectionString = Source=C:\Documents and Settings\nic-15\My Documents\ALRS.mdb") If (errorProvider1.GetError(c).Length > 0) examine the contents of the errorProvider for each control Private void buttonSave_Click(object sender, EventArgs e) But i am unable to solve my problem my code is : ![]() 3/2/2023 0 Comments Kylo ren actor![]() He is a method actor, and would often be seen walking around the Star Wars set wearing his Kylo Ren mask.He hates watching himself on screen, Star Wars: The Force Awakens is one of the only things he’s seen himself in.Adam Driver used to accidentally make classmates cry at drama school with his intensity and the aggression he had to develop in the marines.His prominence in such a legendary franchise has all but ensured him eternal global fame and recognition, and with his proven ability to turn his hand to diverse roles, I predict that we’ll see him in many more blockbuster hits in the future. He also continues to work with his self-founded charity, Arts in the Armed Forces, which provides theatre-based entertainment to the armed forces for their enjoyment and emotional benefit.Īdam Driver has planted himself firmly in the spotlight, and with the return of Kylo Ren being hotly anticipated in the December 2017 release of Star Wars: The Last Jedi, it seems that he isn’t going away anytime soon. Earlier this year he starred as one-armed Clyde alongside Channing Tatum and a unrecognisable Daniel Craig in heist-comedy Logan Lucky, which sees the Logan brothers (Tatum and Driver) attempt to rob the Charlotte Motor Speedway. In 2015’s While We’re Young, Driver teams up with Amanda Seyfried, the two of them playing a hipster young couple who befriend older husband and wife Ben Stiller and Naomi Watts, drawing them away from their boring friends with kids into a more exciting lifestyle.ĭriver has maintained a hectic schedule over the past two years, juggling Star Wars commitments with numerous other films, most notably Martin Scorsese’s Silence and the title role in Paterson, both in 2016. 2014 saw him win the Volpi Cup for Best Actor at the 71 st Venice International Film Festival for his role in Hungry Hearts as Jude, a father desperately trying to protect his child from its mother’s alternative and ultimately dangerous parenting techniques. Several of these were directed by famous names, such as Steven Spielberg’s Lincoln in 2012 and the Coen Brothers’ Inside Llewyn Davis in 2013. Adam Driver is exceptional at portraying his character’s emotional complexity, helping us to understand the impact of the serious issues he deals with, and why he might not always act in the most endearing manner.Īs well as starring in Girls, from 2012 to 2015 Driver also appeared on the big screen in a number of films. Unsurprisingly this doesn’t last and they launch into an on-off scenario, dealing with cheating, mental health breakdowns, and being long distance for a time. By the end of the season, she manages to tie him down, and he slowly morphs into an affectionate, if not slightly clingy boyfriend figure. ![]() ![]() When we meet him in season one, he’s admittedly unlikeable, playing with Hannah’s emotions and refusing to commit to a proper relationship. Throughout the show’s six-season run, aspiring actor Adam exhibited an impressive trajectory of character development. ![]() He played the wonderfully weird, slightly socially inept, but ultimately lovable Adam Sackler, the love interest of Hannah Horvath (Lena Dunham). Edgar, Law & Order, You Don’t Know Jack), Adam Driver landed his breakthrough role in Lena Dunham’s Girls. In 2012, after acting in several off-Broadway shows and supporting parts in both TV and film ( J. ![]() 3/2/2023 0 Comments Pmp aware login![]() ![]() Please refer to your respective state licensing board for additional information regarding the CURES consultation requirement. Section 11165.4 of the Health and Safety Code, sets forth the requirements for mandatory consultation of CURES. Prescribers and Pharmacists can register by accessing the CURES User Registration. Prescriber and Pharmacist registration to access CURES is simple and fully automated. ![]() California licensed pharmacists must register for access to CURES upon issuance of a Board of Pharmacy Pharmacist License. CURES Registration RequirementsĬalifornia law (Health and Safety Code Section 11165.1) requires all California licensed health care practitioners authorized to prescribe Schedule II, Schedule III, Schedule IV and Schedule V controlled substances to register for access to CURES upon issuance of a Drug Enforcement Administration Controlled Substance Registration Certificate. Regulatory agency officials and law enforcement officials may access information in CURES only to assist the efforts of their agencies to control the diversion and resultant abuse of controlled substances. Licensed health care practitioners and licensed pharmacists may access information in CURES only for patients under their care. The controlled substances dispensation history of an individual that is received by a practitioner or pharmacist from CURES is subject to the Confidentiality of Medical Information Act and the Health Insurance Portability and Accountability Act of 1996 (HIPAA), including the HIPAA regulations in 45 Code of Federal Regulations parts 160 and 164.Īccess to CURES is strictly limited. The operation of CURES complies with all applicable federal and state privacy and security laws and regulations. CURES is committed to the reduction of prescription drug abuse and diversion without affecting legitimate medical practice or patient care. Controlled Substance Utilization Review and Evaluation System California’s Prescription Drug Monitoring ProgramĬURES (Controlled Substance Utilization Review and Evaluation System) is a database of Schedule II, Schedule III, Schedule IV and Schedule V controlled substance prescriptions dispensed in California serving the public health, regulatory oversight agencies, and law enforcement. The second sample Delegate Agreement demonstrates an agreement between one Authorizing User and multiple Delegates.ĭetailed information concerning Delegate eligibility and Delegate Agreement requirements are described in DOJ’s October 14, 2022, bulletin entitled, New Features and Requirements for Expanded Delegate Access. The first sample Delegate Agreement demonstrates an agreement between one Authorizing User and one Delegate. For detailed information view the Quick Reference Guide.ĭOJ has also created two sample Delegate Agreements. The Department of Justice (DOJ) has created a Quick Reference Guide that details how to establish and activate a Delegate in the optimized CURES, along with questions and answers. To continue using Delegates after April 11, 2022, new Delegate associations must be established. The optimized CURES includes expanded Delegate functionality, which allows Delegates to directly access data in CURES.Īll prior Delegate associations were disabled upon the release of the optimized CURES on April 11, 2022. New Requirements for Expanded Delegate Functionality ![]() 3/2/2023 0 Comments Layouteditor slanted array![]() ![]() ![]() Transistor arrays were quickly and reliably configured for different logic functions by an additional process step of inkjet printing conductive silver wires and poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) resistors between transistors or between logic blocks. We have fabricated arrays of organic thin-film transistors (OTFTs) and printed electronic components driving an electrophoretic ink display up to 70 mm by 70 mm on a single flexible transparent plastic foil. In this paper, we present a functional integrated plastic system. 2 is the IGA fabricated with TUC process. This structure has been designed and implemented for two different processes of CPI (Center for Process Innovation) and TUC (Technical University of Chemnitz) according to their Design Rules (DRC) restrictions and process yield. 1 in which pseudo ratioed pMOS with three power rails (VDD, GND, VSS) is selected as the cell style in basic bulk cell, for a process with only p-channel transistors available. In this methodology, IGA is divided into main pCells such as (Basic-Bulk-Cell (repetition of OTFTs), Wire-Cell, Inter-Connection Cell and PAD cells) and the design parameters of the pCells vary according to any target process restrictions. ![]() Furthermore, since there is a big variety of printed electronics process technologies, Paramiterizable-Cell (PCell) approach is used to scale the inkjet gate array regardless of the technology dimensions (design rules). So, depending on the number of KGOs and their position and the target function, each fabricated foil can have its own personalization. This solution is achieved by wiring only the Known Good OTFTs (KGO) which has been characterized and marked individually in the GA customization stage. In order to overcome this issue, fault-tolerant concept is proposed as a strategy to allow achieving high yield on circuits out of foils with mid yield in Organic Thin Film Transistors (OTFTs). The main barrier for the wide adoption of printed electronic circuits is its poor yield. In this paper, we introduce Technology-Independent Fault-Tolerant Inkjet Gate Array (IGA) structure as a novel concept to allow ASPEC design in Organic Electronics. Application Specific Printed Electronic Circuits (ASPEC), are circuits designed and customized for a special application, (the equivalent term for ASIC in Organic Electronics). ![]() ![]() ![]() Summit White Aero Pack Sat Nav Infinity Sound Upgrade Full Leather AFL's Tyre Pressure Monitoring System How she looked when I picked her up: Then the cosmetic mods started! 1st Mod Interior bulbs changed for LED's 2nd Mod Number plate LED's 3rd Mod Heko wind deflectors 4th Mod. It's like 0.6 because the glass needs interior which i can't do. Pirelli zeros from the stock cars are on it. Its basically a Brazilian stock car full fat but with the power lowered with a pretty cute body plonked on top. ![]() Its a silhouette car so if it doesn't drive like your Astra from 2007, I'm sorry, it won't. For improved handling, the ride height is lowered by 15 mm and wheelbase. modifications, troubleshooting, maintenance, and more! But first comes the chassis, which in this GTC differs from the standard 5-door Astra model. Our Vauxhall performance chips and Vauxhall remapping via the OBD port will dramatically improve your car's performance and improve your MPG! Fantastic drivability - 15-35% extra power Be happy at the pumps - 8-15% improved mpgVauxhall Astra Gtc Limited Edition 3 Door 2017-2019 Rear Bumper Genuine Buy high quality, genuine rear bumper for a. Do you want to gain more power, torque and better MPG for your Vauxhall Astra? Then look no further. ancak devir ve hız arttıkça yayılan sesler, sinirli ve yüksek gümbürtülere dönüşüyor. orta devirlere kadar motor, boğuk ve barışçı bir sesle çalışıyor. 0-100 km/s hızlanmayı astra gtc, 7.5 saniyede gerçekleştirirken, 234 km/s'lik son hızına çok da uzun olmayan bir sürede ulaşabiliyor. Typical stage 2 mods often include: high flow fuel injector, Fast road cam, fuel pump upgrades, Ported and polished head, Power/Sport clutch. El calatrava futbolero, al menos os dejan campo 0, 20:50 #16: Chabelita. It was first launched in September 1991 as a direct replacement to the Opel Kadett.As of 2022, the car slots between the smaller Corsa supermini and the larger Insignia large family car. Reviews ( 1) Description …The Opel Astra is a compact car/small family car developed and produced by the German automaker Opel since 1991, currently at its sixth generation. Tags: Germany 2.4L inline-4 16v twin-screw supercharger turbo 6-speed manual FWD street/trackday. Opel Astra H Sofa Cama, Car Mods, Caravan, Chevrolet, Insta, Bmw.2012. Reč je o Astri J GTC sa fiatovim 2.0 …Explore Damir Cesnak's board "Opel Astra GTC" on Pinterest. Always disable your anti virus before extracting the game to prevent it from deleting the crack files.Opel Astra H Gtc Opc 1 31 X Ets2 Mods Euro Truck Simulator 2 Ets2mods Lt Opel Astra J Interior V1 5 By Trzpro 1 39 X For Ets2 What Is The Best Iphone Vent Holder Astra Owners Network Opel Astra J Interior V1 5 By. Also, be sure to right click the exe and always select “Run as administrator” if you’re having problems saving the game. Look for a ‘HOW TO RUN GAME!!.txt’ file for more help. You need these programs for the game to run. If you get any missing dll errors, make sure to look for a _Redist or _CommonRedist folder and install directx, vcredist and all other programs in that folder. NOTICE: This game is already pre-installed for you, meaning you don’t have to install it. It is the full version of the game. Don’t forget to run the game as administrator.Įuro Truck Simulator 2 (v1.45.3.0s & ALL DLC)
3/2/2023 0 Comments Draftsight 2016 mill![]() ![]() Download Guide Configuration & Basic Customization This document provides information on how to customize the DraftSight User Interface and program environment. Many variables are customized per the riders needs. This is a quick reference guide to help when installing and activating the DraftSight Standard, Professional, or Premium software. a lot of stuff!), Diamond frames are laid out in Bike Cad, a computer drafting program by Brent Curry. ![]() Once I know who I am building for (body measurements, needs, desires, terrain. Modern steel is light and has all the weight, flex and strength characteristics desirable in bicycle performance and is perfect for the Farfarer trailer design. The Farfarer trailers are now built exclusively with Tange Tubing, of the highest quality. Frame fittings components come from Paragon Machine Works (the best) as well as other suppliers and many small parts are made here in the shop. Current framesets are a mixture of Columbus, Vari-Wall and Tange Tubes- all very high quality. That said, I am near the end of my my supply of True Temper tubing (out of business for a few years now) and the newer Vari-Wall butted tubes from Ohio can be hard to come by. It is much the same romanticism that draws me to bicycles. Since AutoCAD 2016 you can also use the osnap mode geometric center to snap to the center of gravity of an object - e.g. It is a care for energy, efficiency, and conservation. ![]() To be clear, my desire to use American-sourced materials and parts does not stem from nationalist inclinations, but from the physical realities of geography and efficiency. While I have prioritized American-made materials and components, it is not always possible. I also build the Farfarer Trailer, the Frances Spatula, and more. Windows DraftSight DraftSight (64-bit) Free to try Create, edit, view, markup, and. Created for individuals, designers, companies, manufacturers and makers. Showing 1-10 of 5,073 Results for 'draftsight 2016' DraftSight Free Create, edit, and view DWG files. In my compact framebuilding shop (235 square-feet), I build bicycles from the highest quality materials designed to the rider’s body measurements and the bike's intended use. DraftSight Premium 599 USD / yr The robust 2D drafting and 3D design experience with full 3D capabilities and constraints features to help meet all of your drafting, modeling, prototyping, manufacturing, laser cutting and 3D printing needs. ![]() 3/2/2023 0 Comments Intervalo de contorno![]() ![]() ![]() Moreover, it showed a shift towards neokestose, which represented ~27% of total FOS, and yielded 31.8 g l-1 blastose by using glucose as exclusive fructosyl-acceptor. Interestingly, N254T variant enhanced the wild-type protein production of fructosyl-erythritol and FOS by `30% and 48%, respectively. Complexes with fructosyl-erythritol or sucrose were analyzed by crystallography and the effect of mutational changes in positions Gln-176, Gln-228, and Asn-254 revised to explore their role in modulating this biocatalytic process. In this work, the structural determinants of Ffase involved in the transfructosylating reaction of the alditols mannitol and erythritol have been studied in detail. The β-fructofuranosidase from Schwanniomyces occidentalis (Ffase) is a useful biotechnological tool for the fructosylation of different acceptors to produce fructooligosaccharides (FOS) and fructo-conjugates. The results presented here highlight the relevance of the pair Gln-228/Asn-254 for Ffase donor-sucrose binding and opens new windows of opportunity for optimizing the generation of fructosyl-derivatives by this enzyme enhancing its biotechnological applicability. Noteworthy, N254D and Q176E replacements turned the specificity of Ffase transferase activity towards the synthesis of the fructosylated polyols at the expense of FOS production, but without increasing the total reaction efficiency. Moreover, it produced neokestose, which represented $$\sim$$Ģ7% of total FOS, and yielded 31.8 g l−1 blastose by using glucose as exclusive fructosyl-acceptor. Interestingly, N254T variant enhanced the wild-type protein production of fructosyl-erythritol and FOS by $$\sim$$ģ0% and 48%, respectively. ![]() Complexes with fructosyl-erythritol or sucrose were analyzed by crystallography and the effect of mutational changes in positions Gln-176, Gln-228, and Asn-254 studied to explore their role in modulating this biocatalytic process. AbstractThe β-fructofuranosidase from Schwanniomyces occidentalis (Ffase) is a useful biotechnological tool for the fructosylation of different acceptors to produce fructooligosaccharides (FOS) and fructo-conjugates. ![]() |
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